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Development of a new contactless dielectrophoresis system for active particle manipulation using movable liquid electrodes

Authors
Gwon, Hyuk RokChang, Suk TaiChoi, Chang KyoungJung, Jung-YeulKim, Jong-MinLee, Seong Hyuk
Issue Date
7월-2014
Publisher
WILEY
Keywords
Active particle manipulation; Contactless dielectrophoresis; Movable liquid electrode
Citation
ELECTROPHORESIS, v.35, no.14, pp 2014 - 2021
Pages
8
Journal Title
ELECTROPHORESIS
Volume
35
Number
14
Start Page
2014
End Page
2021
URI
https://www.kriso.re.kr/sciwatch/handle/2021.sw.kriso/851
DOI
10.1002/elps.201300566
ISSN
0173-0835
1522-2683
Abstract
This study presents a new DEP manipulation technique using a movable liquid electrode, which allows manipulation of particles by actively controlling the locations of electrodes and applying on-off electric input signals. This DEP system consists of mercury as a movable liquid electrode, indium tin oxide (ITO)-coated glass, SU-8-based microchannels for electrode passages, and a PDMS medium chamber. A simple squeezing method was introduced to build a thin PDMS layer at the bottom of the medium chamber to create a contactless DEP system. To determine the operating conditions, the DEP force and the friction force were analytically compared for a single cell. In addition, an appropriate frequency range for effective DEP manipulation was chosen based on an estimation of the Clausius-Mossotti factor and the effective complex permittivity of the yeast cell using the concentric shell model. With this system, we demonstrated the active manipulation of yeast cells, and measured the collection efficiency and the dielectrophoretic velocity of cells for different AC electric field strengths and applied frequencies. The experimental results showed that the maximum collection efficiency reached was approximately 90%, and the dielectrophoretic velocity increased with increasing frequency and attained the maximum value of 10.85 +/- 0.95 mu m/s at 100 kHz, above which it decreased.
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해양공공디지털연구본부 (해사안전·환경연구센터)
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