Deep learning based lens free shadow imaging platform (Cellytics)

Abstract

We developed a deep learning based LSIT decision algorithm on the Alexnet model, a convolutional neural network, and applied to the Grad-CAM, a visual explanation algorithm via gradient-based localization (Fig. 1). And we also developed LSIT platform (Cellytics) along with dedicated cell chip, enabling automatic collection of shadow images of various micro particles and cells (Fig. 2). Shadow images of different sizes of polystyrene micro-beads (5, 10, and 20 um) and human leukocytes (CD4, CD8) were recorded with Cellytics and set to test the decision algorithm.