발광박테리아를 이용한 생태독성 감시장치 개발

Abstract

Ecotoxicity monitoring device by using Vibrio fischeri was evaluated for the purpose of monitoring the leakage of Hazardous and Noxious Substance (HNS). The prototype of ecotoxicity monitoring device with two separate reactors was used to measure Toxic Index(TI) of Vibrio fischeri. Control and sample were placed in each reactor and then measured the intensity of luminousness after 15 minutes. According to the water pollution standard method, zinc sulfate heptahydrate was selected for reference material. 98.0~98.7% of TI was measured at 19.67mg/L of Zinc sulfate which is twice the expected EC50. 94.8~96.2%, 28.6~29.1%, 1.9~3.8% of TI were measure at 9.67mg/L, 7.25mg/L, 4.84mg/L of zinc sulfate. Ecotoxicity monitoring device indicated high sensitivity compressed to laboratory analysis. The results showed possibility of online ecotoxicity monitoring systems for marine industrial facilities and ship accident. Developing monitoring device with additional reactors is expected to dilute samp